SD Folders & Root Access

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http://androidforums.com – Here's a hypothetical situation that I'm trying to figure out: Step 1: Buy a new phone, unlock the bootloader, root it, and put a custom recovery (TWRP or CWM) on it. Step 2: Make a full backup (presumably saved in TWRP or CWM folder on SD) Step 3: Revert back to stock and unroot the device. Step 4: Re-root the device and reinstall a custom recovery. After step 4, would that original backup reappear or did it get wiped upon flashing the stock factory image and unrooting the device? Thanks in advance. (General)