Remove domain user access to ubuntu machine

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http://askubuntu.com – I have configured my Ubuntu server to allow login using Windows AD credentials. I have added enabled a domain group to login to ubuntu machine(by adding them in sudoer file) #%mydomain\\domain^users ALL=(ALL) ALL Now I want to remove the login access of these group members to the machine. I tried modifying sudoer(removed #%mydomain\\domain^users ALL=(ALL) ALL ) But still the users of that group is able to login. How can I remove the access. (HowTos)