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Quantifying the effects of partition mis-alignment

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http://serverfault.com – I'm experiencing some significant performance issues on an NFS server. I've been reading up a bit on partition alignment, and I think I have my partitions mis-aligned. I can't find anything that tells me how to actually quantify the effects of mis-aligned partitions. Some of the general information I found suggests the performance penalty can be quite high (upwards of 60%) and others say it's negligible. What I want to do is determine if partition alignment is a factor in this server's performance problems or not; and if so, to what degree? So I'll put my info out here, and hopefully the (HowTos)